Contractile function and myocardial metabolism in early period after maximally allowable liver resection in rats

Abstract

The purpose: to evaluate the contractility and myocardial metabolism in the early period after the maximally allowable liver resection in the experiment on isolated hearts. Methods. Direct assessment of myocardial contractile function and metabolism of isolated isovolumic hearts was carried out in the early period after the maximally allowable 80% liver resection in the experiment on 64 outbred albino male rats. At different times after surgery (1, 3, 6, 12 hours and 1, 3, 7 days) we opened the chest, took heart, retrograde perfusion of the hearts was made through the aorta with a solution of Krebs-Henseleit in the mode of constant pressure. Based on the analysis of the pressure curve in the left ventricle systolic, diastolic and developed pressure, rate of contraction and rate of relaxation were calculated. For evaluation of the functional reserves of the myocardium hypoxic perfusion was used. Simultaneously with the registration of pressure we took samples of the perfusate passing through the coronary vessels, determined the content of glucose, lactate, activity of aspartataminotranferase. Results. It is revealed that in the first hours after surgery developed acute of systolic-diastolic left myocardial dysfunction, with prevalence of its diastolic component. Hearts of operated animals were less resistant to hypoxic and reoxygenation damage. A violation of carbohydrate metabolism and bioenergetics in the myocardium had been after the operation. Hearts of animals of experimental groups consumed more glucose per unit of the function, allocate more lactate, increased leakage of aspartateaminotransferase in coronary flow was determined, indicating increased permeability and destruction of the membranes of cardiomyocytes. Conclusion. Thus, inhibition of the contractile function of the myocardium, especially its relaxation, hypoxia of the myocardium, inhibition of energy metabolism, mitochondrial dysfunction and damage of the membranes of cardiomyocytes were in the early period after the maximally allowable liver resection.