Analysis of expression C3aR, C5aR1 (SD88) on myeloid cells in patients with sepsis

Abstract

The purpose. Аnalyze the importance of the level of expression S3aR, S5aR (SD88) on monocytes and neutrophils, myeloid series in patients with sepsis. Anaphylatoxins C3a and C5a act as one of the major mediators of inflammation by interacting with effector cells through C3aR and C5aR (CD88) when septic conditions occur. Expression C3aR, C5aR (CD88) on the cells of the myeloid series is an important pathogenetic link in generalized inflammation. Methods. The study included 32 patients and the level of expression С3aR, С5aR1(СD88) by flow cytometry on monocytes and neutrophils was examined in patients with sepsis, depending on the severity of the disease. Results. Decreased expression of the receptor C5aR1(CD88) on monocytes and neutrophils was seen in patients with sepsis. Differences in expression of the receptor C3aR with decline of neutrophils and higher rates on monocytes compared to the control group were revealed during the observation. The degree of expression on monocytes С3aR directly correlated with the degree of severity in patients with surgical sepsis. Conclusion. The study of this marker can be used as a predictor of organ dysfunction the degree of severity in patients with sepsis.