Estimation of atherogenic immune complexes containing modified lipoproteins in complement fixation tests

  • B. B. Shoibonov Scientific-Research Institute of Normal Physiology named after P.K. Anokhin, Moscow, Russian Federation
  • M. A. Kravchenko Center Research of Neurology, Moscow, Russian Federation
  • V. Y. Baronets The National Research Center of Addictions, Moscow, Russian Federation
  • S. M. Tolpygo Scientific-Research Institute of Normal Physiology named after P.K. Anokhin, Moscow, Russian Federation
  • M. V. Kostyreva Center Research of Neurology, Moscow, Russian Federation
  • A. A. Shabalina Center Research of Neurology, Moscow, Russian Federation
  • T. S. Zamolodchikova Scientific-Research Institute of Normal Physiology named after P.K. Anokhin, Moscow, Russian Federation
  • A. V. Kotov Scientific-Research Institute of Normal Physiology named after P.K. Anokhin, Moscow, Russian Federation
  • L. F. Panchenko The National Research Center of Addictions, Moscow, Russian Federation; Scientific Research Institute of General Pathology and Pathophysiology, Moscow, Russian Federation
Keywords: atherosclerosis, immune complexes containing mmLDL, cholesterol in immune complexes, extent of binding of the complement, immune complexes atherogenicity

Abstract

A method for determining atherogenicity of the immune complexes containing multiple modified low-density lipoprotein (mmLDL) in complement fixation test has been found. In the proposed method, the precipitate immune complexes containing mmLDL (IC mmLDL) was prepared from human serum by treating it with buffer (8.3% of th PEG 3350 and 3.3% PVP 12600 th in the ratio 1: 1.2) for 10 min at 23°С. IC mmLDL aggregates were separated by centrifugation at 3100g for 10 min at 23°С. The precipitate IC mmLDL was dissolved in buffer without PEG and PVP, cholesterol content and the degree of binding of guinea pig complement were measured. Atherogenicity of the IC mmLDL was registered as the ratio of the degree of complement binding to cholesterol in the the immune complexes.

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Published
2014-12-05
How to Cite
Shoibonov B. B., Kravchenko M. A., Baronets V. Y., Tolpygo S. M., Kostyreva M. V., Shabalina A. A., Zamolodchikova T. S., Kotov A. V., Panchenko L. F. Estimation of atherogenic immune complexes containing modified lipoproteins in complement fixation tests // Patologicheskaya Fiziologiya i Eksperimental’naya Terapiya (Pathological physiology and experimental therapy). 2014. VOL. 58. № 4. PP. 133–138.
Issue
Vol. 58 No. 4 (2014)
Section
Methods