The effect of low frequency electrical stimulation on bone tissue regeneration

Abstract

The study was performed on 30 male rats of Wistar line (weight 330 - 360 g, age 3.5 months). In an experimental model of damage to the femur bone in the hip joint studied the effect of low frequency electrical stimulation of the damaged area on the rate of regeneration of bone. The animals were divided into two groups. Control (15 rats) and experienced (15 rats). In the experimental animals underwent stimulation of the injury site for 5 min daily for 7 days, 14 days and 21 days. Stimulation was carried out using a device "Osteon-1" generating a mixed signal of two voltage pulse of varying duty cycle, one of which is modulated to a higher frequency. Signals were not synchronized with respect to each other, unipolar with varying frequencies and amplitudes. The obtained results show the effectiveness of the electrical stimulation currents of low frequency in the restoration of bone tissue after damage. Morphological studies showed that electrical stimulation to accelerate the regeneration of damaged bone at all stages of the study (7,14,21 day), causes a more pronounced integration of newly formed bone with the old intact bone and promote the formation of more powerful periosteal calluses in comparison with the control.