Registration of the protein in the serum with a field-effect nanotransistor biosensor

  • Yu. D. Ivanov Institute of Biomedical Chemistry, Moscow, Russia; TSAMAX Ltd., Moscow, Russia
  • К. А. Malsagova Institute of Biomedical Chemistry, Moscow, Russia
  • Т. О. Pleshakova Institute of Biomedical Chemistry, Moscow, Russia
  • I. D. Shumov Institute of Biomedical Chemistry, Moscow, Russia
  • A. L. Kaysheva Institute of Biomedical Chemistry, Moscow, Russia
  • V. P. Popov Institute of Semiconductor Physics, SB RAS, Novosibirsk, Russia
  • O. V. Naumova Institute of Semiconductor Physics, SB RAS, Novosibirsk, Russia
  • B. I. Fomin Institute of Semiconductor Physics, SB RAS, Novosibirsk, Russia
  • D. A. Nasimov Institute of Semiconductor Physics, SB RAS, Novosibirsk, Russia
  • A. V. Latyshev Institute of Semiconductor Physics, SB RAS, Novosibirsk, Russia
  • A. L. Aseev Institute of Semiconductor Physics, SB RAS, Novosibirsk, Russia
  • V. Yu. Tatur TSAMAX Ltd., Moscow, Russia
  • N. D. Ivanova Skryabin Moscow State Academy of Veterinary Medicine and Biotechnology, Moscow, Russia
  • G. M. Konovalova Sochi State University, Sochi, Russia
  • A. I. Archakov Institute of Biomedical Chemistry, Moscow, Russia
Keywords: the field transistor; detection of albumen; biologysensor

Abstract

A method for detection of cancer-associated protein D-NFATc1 in serum using nanowire (NW) biosensor based on field-effect nanotransistor is developed. Field-effect nanotransistor was fabricated on the basis of “silicon-on-insulator” structures. For the biospecific detection of target protein, the NW surface was modified with aptamers against the target protein. Using the 3 um-NW enabled to obtain stable source-drain characteristics and to register D-NFATc1 in serum at concentration of 2.5×10-14 M in the mode of drain-source current vs. gate voltage characteristics measurements. Data collection in the mode of drain-source current vs. gate voltage characteristics measurements was carried out with the use of high-speed data collection system running TURBO NBS software.

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Published
2016-03-22
How to Cite
Ivanov Y. D., Malsagova К. А., Pleshakova Т. О., Shumov I. D., Kaysheva A. L., Popov V. P., Naumova O. V., Fomin B. I., Nasimov D. A., Latyshev A. V., Aseev A. L., Tatur V. Y., Ivanova N. D., Konovalova G. M., Archakov A. I. Registration of the protein in the serum with a field-effect nanotransistor biosensor // Patologicheskaya Fiziologiya i Eksperimental’naya Terapiya (Pathological physiology and experimental therapy). 2016. VOL. 60. № 1. PP. 94–98.
Issue
Vol. 60 No. 1 (2016)
Section
Methods