Method for measurement of circulating multiply modified low-density lipoprotein in the blood serum

Abstract

The purpose of research was development of method for the measurement of naturally occurring multiply modified LDL (nomLDL) in serum. nomLDL are atherogenic components of patients with cardiovascular diseases serum. Results. We have developed a lectin-sorbent assay for the determination of desialylated LDL in serum. The assay is based on the binding of desialylated LDL by immobilized Ricinus communis agglutinin with subsequent measurement of lipoprotein through use of anti-apolipoprotein (apo) B antibody. Conclusion. Developed method for measurement of circulating multiply modified low-density lipoprotein in the blood serum characterized with high accuracy and reproducibility and may help establish the diagnostic value of this lipoprotein as a risk factor of atherosclerosis.