Protein and nitrogen metabolism in the liver of mice during recovery period after skeletal injury

Abstract

Purpose of study. Study the dynamics of protein and nitrogen metabolism in the recovery period after tibia fracture and after fracture of tibia bones in mice against a background of acute liver failure. Methods. Experiments were conducted on 134 male CBA mice at the age of two months of postnatal period, weighing 25-30 grams. In the first group were simulated fractured shin bones by mechanical damage to the limb segment in the upper third of the medial surface; in the second group acute liver failure was simulated by intraperitoneal injection of 20% carbon tetrachloride solution in olive oil; in the third group was simulated acute liver failure, then on the 3 day – tibia fracture. It was studied content of total protein in liver and blood serum, urea, alanine and aspartate, chemical composition of the bone of the injured limb segment in 3, 7, 28 days after tibia fracture and after fracture of tibia bones against a background of acute liver failure intoxication by carbon tetrachloride. Results. It was found a decrease bone tissue collagen, reduction of total protein level and urea in the liver in early posttraumatic period, reduction in the concentration of total serum protein in mice after tibia bones fracture against acute liver failure. It was noticed increase of total protein and urea in early posttraumatic period in mice without acute liver failure. Conclusion. It was found that in the recovery period changes in the indices of protein-nitrogen metabolism in the liver induced by tibia fractures are a part of the adaptive restructuring aimed at maintaining the protein levels in the serum. Deepening of functional disorders of the liver under the influence of two damaging factors (intoxication and trauma) can cause disturbances of the bone metabolism.