Change of total nonsaturation of blood plasma lipids for patients with arterial hypertension in glucose tolerance test

Abstract

The research purpose – definition in dynamics of the glucose tolerance test (oGTT) in blood plasma for patients with arterial hypertension of the total content of double bonds (DB) and non-esterified fatty acids (NEFA), of the content of individual fatty acids (FA), glucose, insulin and other analytes for clarification of functional communications between them. Methods. The oral glucose tolerance test (oGTT) was carried out for 20 patients with arterial hypertension at absence and presence of an insulin resistance (IR) syndrome. Blood was taken from a vein on an empty stomach and in 2 and 4 hours, blood plasma was separated from erythrocytes and stored at-70ºС. Quantitative definition of FA was carried out on the analytical gas chromatograph with use of standard FA samples. The total double bonds content in a lipid pool of blood plasma was determined by an ozonization method. Content of insulin and C-peptide in blood plasma was determined on the Immulayt analyzer and glucose, TG, NEFA, free cholesterol (TCh) and other analytes - on the biochemical analyzer of the Arkhitekt-800 model. Reliability of distinction of sizes was considered by t-criterion, using 95% confidential probability (p≤0,05). For patients with various resistances to insulin (IR) the plasma content of NEFA decreases 3 times. Results. It is established that for patients with various resistances to insulin (IR) the plasma content of NEFA decreases 3 times. Out of IR insulin secretion during 2 hours after glucose loading increases 3 times, and decrease in the individual FA content is greater; in IR insulin secretion increases 8-fold, and decrease in individual FA content is less pronounced. Effect of insulin reflects a decrease of the DB (total nonsaturation of blood plasma lipids) and FA (olein and linoleic) content in blood plasma - blockade of triglyceride hydrolysis in subcutaneous adipocytes. Reliable correlation (r=0,851) between the DB content determined by an ozonization method and calculated proceeding from concentration of individual FA with known DB number is revealed. Conclusion. The received results demonstrate that effect of insulin can be estimated at GTT authentically on the basis of change in blood plasma not only of the glucose content, but also concentration of nonsaturated FA and DB number characterizing degree of nonsaturation of blood plasma lipids.