The influence of acute or chronic bankol intoxication of the lipid peroxidation processes and the activity of antioxidant enzymes

Abstract

The purpose is the investigation of the influence of acute and chronic bancol intoxication on lipid peroxidation processes and antioxidant enzymes activity and search of methods to correct the unfavorable action of investigated insecticide. Methods. Experiments were carried out on Vistar albino rats (n=32, 180-220g). For modeling bancol intoxication we used models of acute and chronic intoxication: acute - intragastric injection of bancol in a dose 1/50 LD50 (4,42 mg per animal) during 3 days daily; chronic – injection of bancol daily during 28 days intragastrically in same dose. Animals were divided into four groups: naïve control, acute bancol intoxication, chronic bancol intoxication, chronic bancol intoxication + mexidol. Rats received intramuscular mexidol during 14 days (15-28 days of bancol injection) in a dose 50 mg per animal. We determine content of acylhydroperoxides (AHP), malonic dialdehyde (MDA), activity of superoxiddismutase (SOD) and catalase, which were analyzed spectrophotometrically using of standardized methods. Results. It was established that acute and chronic bancol intoxication is accompanied with the increase of AHP and MDA content in plasma and liver tissue, increase of both enzymes activity in plasma and its decrease in liver tissue. The administration of mexidol caused the decrease of lipid peroxidation, increase of SOD and catalase activity in liver. Conclusion. The results obtained were shown the activation of lipid peroxidation in bancol intoxication and antioxidant effect of mexidol.