Eefect of vancomycin on migrating myoelectric complex and intestinal microflora

Abstract

The purpose. To study the effect of vancomycin on myoelectric activity of small intestine and species composition of the intestinal microbiota in healthy rats. Methods. The studies were performed on 14 male Wistar rats weighing 250-300 g. Three electrodes were implanted in the muscle layer of the small intestine and the probe was implanted in antral part of the stomach. Myoelectric recordings were measured from unanesthetized rats before and after administration of vancomycin (during 7 consecutive days). Jejunum, cecum and feces samples were cultured quantitatively. Results. Myoelectric recordings in vancomycin rats showed longer intervals between activity fronts of the migrating myoelectric complex (MMC) (phase III) compared with background recordings. In addition, number of propagating complexes was dramatically decreased. The duration of phase II was longer, and the duration of phase I was shorter than that of normal animals. It was identify an increase in species diversity of microorganisms in the jejunum and decreased it in the cecum and feces, as well as a moderate decrease in the number of members of the normal microflora in the cecum and feces and appears opportunistic gram-negative flora in the proximal jejunum. Conclusion. Thus, disruption of MMC with vancomycin promotes bacterial overgrowth that partly bound, apparently with the migration of the fecal microflora in the proximal jejunum.