Effect of potassium comenate on antioxidant glutathione system in brain of stressed mice

Abstract

The purpose. This work is devoted to study of potassium comenate influence on the content of reduced glutathione, glutathione peroxidase and glutathione reductase activity in the brain of mice exposed to immobilization stress. Methods. The experiments were performed on 62 white mongrel male mice weighing 23-25 g. Immobilization stress in mice induced by suspending them for cervical crease for five hours. After 5 hours the mice were decapitated, the brain was placed in liquid nitrogen and then in the brain tissue was determined by the content of reduced glutathione (GSH), the activity of glutathione peroxidase (GPO) and glutathione reductase (GR). Potassium comenate administered intragastrically (gavage) in doses of 2, 4 and 8 mg/kg body weight, before of stressing, fasting for three days, once per day. The animals were divided into groups: control; treated with potassium comenate; stressed; stressed treated with potassium comenate. Results. It is found that the use of potassium comenate at doses of 4 and 8 mg / kg contributes to maintaining, GPO and GR activity and GSH content in the brain of stressed mice to intact control levels. That is, there is the maintenance of the resource of the antioxidant glutathione system at the level of the physiological norm, indicating a pronounced antioxidant, stress-protective activity of potassium comenate. The dose of 2 mg / kg contributed to keeping the content of GSH at the physiological norm, but the activity of the GR and GPO had no significant effect. Conclusion. Prestress application of potassium comenate in doses of 4 and 8 mg / kg retains GR and GPO activity and GSH content in the brain of stressed mice at physiological norm that indicates its antioxidant, stress-protective activity.