The technology of obtaining multipotent spheroids from limbal mesenchymal stromal cells for reparation of injured eye tissues

  • Nastasia V. Kosheleva Institute of General Pathology and Pathophysiology, Moscow; Faculty of Biology, Lomonosov Moscow State University, Moscow http://orcid.org/0000-0002-2665-4972
  • Irina M. Saburina Institute of General Pathology and Pathophysiology, Moscow; Russian Medical Academy of Postgaduate Education, Moscow http://orcid.org/0000-0003-2014-2535
  • Irina M. Zurina Institute of General Pathology and Pathophysiology, Moscow http://orcid.org/0000-0002-3275-0215
  • Anastasia A. Gorkun Institute of General Pathology and Pathophysiology, Moscow http://orcid.org/0000-0001-5859-812X
  • Sergey A. Borzenok Fyodorov Eye Microsurgery Complex, Moscow, Russian Federation
  • Denis A. Nikishin Faculty of Biology, Lomonosov Moscow State University, Moscow, Russian Federation; Koltzov Institute of Developmental Biology of Russian Academy of Sciences, Moscow, Russian Federation http://orcid.org/0000-0002-9238-9206
  • Tamara D. Institute of General Pathology and Pathophysiology, Moscow; Russian Medical Academy of Postgraduate Education, Moscow, Russian Federation
  • Ekaterina E. Ustinova Institute of General Pathology and Pathophysiology, Moscow
  • Vadim S. Repin Institute of General Pathology and Pathophysiology, Moscow; Russian Medical Academy of Postgraduate Education, Moscow; Fyodorov Eye Microsurgery Complex, Moscow, Russian Federation
Keywords: 3D culture, cell spheroids, technology of obtaining spheroids, limbal multipotent mesenchymal stem cells (L-MMSC).

Abstract

It is known that stem and progenitor cells open new possibilities for restoring injured eye tissues. Limbal eye zone, formed mainly by derivatives of neural crest, is the main source of stem cells for regeneration. The current study considers development of innovative technology for obtaining 3D spheroids from L-MMSC. It was shown that under 3D conditions L-MMSC due to compactization and mesenchymal-epithelial transition self-organize into cellular reparative modules. Formed L-MMSC spheroids retain and promote undifferentiated population of stem and progenitor limbal cells, as supported by expression of pluripotency markers – Oct4, Sox2, Nanog. Extracellular matrix synthetized by cells in spheroids allows retaining the functional potential of L-MMSC that are involved in regeneration of both anterior and, probably, posterior eye segment.

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Published
2016-11-21
How to Cite
Kosheleva N. V., Saburina I. M., Zurina I. M., Gorkun A. A., Borzenok S. A., Nikishin D. A., Tamara D., Ustinova E. E., Repin V. S. The technology of obtaining multipotent spheroids from limbal mesenchymal stromal cells for reparation of injured eye tissues // Patologicheskaya Fiziologiya i Eksperimental’naya Terapiya (Pathological physiology and experimental therapy). 2016. VOL. 60. № 4. PP. 160–167.
Issue
Vol. 60 No. 4 (2016)
Section
Methods