Assembly of stress granules by personalized endothelial cells HUVEC

Abstract

Abstract:

Stress granules (SGs) are intracellular membraneless organelles formed in response to adverse non-physiological conditions. SGs are temporary ribonucleoprotein particles containing non-translatable mRNAs. One of their functions is transcript preservation during stress response. Most of the current SGs studies employ both model systems – immortalized cell lines and highly specific inducers, which generally do not affect cells in vivo in pathology development. In this article we extrapolated data, previously collected by other research groups on non-pooled untransformed umbilical vein endothelial cells HUVEC. We also studied the ability of pathology associated (e.g. atherosclerosis, salt hypertension) stress-inducers to cause cytoplasmic stress granule formation. Oscillatory shear, hyperosmolarity and cold shock were therefore evaluated.