Reactive oxygen species and high-nitrogen compound in the mechanism of protection and damage in white blood cells in pulmonary tuberculosis

Abstract

The purpose. The aim of the study was to determine changes in the level of reactive oxygen species (ROS) and high-nitrogen compounds (BCA) of white blood cells of patients with various forms of pulmonary tuberculosis, and attempt to identify the pathogenic importance marked disturbances in protection mechanisms and damaged by the disease. Methods. A prospective study of 92 people, who were divided into 4 groups: I- 25 patients with newly diagnosed infiltrative tuberculosis and localization process within the same lobe of the lung, II- 42 patients with newly diagnosed pulmonary tuberculosis with a limited process (tuberculoma), III- 15 patients with fibrosis – cavernosis pulmonary tuberculosis with disease duration of less than 2 years old and group IV – healthy people - 10. Results. It has been found that chronic TB infection suppresses the phagocytes activity of leukocytes in any form of study of its development. However, in more severe forms of tuberculosis with a predominance of exudative tissue reactions (infiltrative) levels of these metabolites in the white blood cells increases and they become damaging significance. With limited forms of pulmonary tuberculosis – tuberculoma- levels of these metabolites (especially ICA) in white blood cells is reduced, which may have a protective value. Conclusion. The obtained results allow to claim that in the diagnosis and assessment of the nature of the tuberculosis process may be used definition of functional and metabolic characteristics of blood leukocytes and plasma (phagocytosis, peroxide, total lipid, BURST TEST, AFC, ICA and their derivatives), as well as the number of amino acids, taurine and arginine in plasma and leukocytes.