Oxidative stress and parameters of spermatogenesis in men with idiopathic infertility before and after the treatment with ethylmethylhydroxypyridine malate
Abstract
Abstract. Oxidative stress (OS) is now recognized as an important factor contributing to the development of various forms of male infertility, including idiopathic. Modern studies are characterized by high heterogeneity and convincing conclusions regarding the effect of antioxidants (AO) on ejaculate parameters have not yet been presented. Aim of the study: to evaluate changes in the main parameters of the ejaculate and the state of the LPO-AOP system when using ethylmethylhydroxypyridine malate in men with idiopathic infertility. Methodology. A retrospective analysis of the results of examination of men (n=225) from infertile couples was carried out and men with idiopathic infertility were identified. A control group of practically healthy men with realized reproductive function was formed. Ejaculate studies were carried out in accordance with WHO recommendations (2022). Determination of indicators of the lipid peroxidation system and antioxidant protection was carried out in the ejaculate using generally accepted spectrophotometric foluorometric methods. Results. After a month of taking the drug ethylmethylhydroxypyridine malate, an increase in the number of sperm with progressive motility was found in the sperm of infertile men, in contrast to patients who did not take this drug. In the ejaculate of men who did not take the antioxidant drug, no significant changes in the indicators of the LPO-AOP system were detected. After taking an antioxidant drug in the ejaculate of men with idiopathic infertility, a statistically significant decrease in LPO indicators is observed against the background of an increase in the power of the antioxidant system, expressed in a significant increase in the level of SOD and AOA. Conclusion. Thus, as a result of the study, it was established that the antioxidant drug ethylmethylhydroxypyridine malate has an activating effect on the concentration and motility of sperm, and also reduces oxidative processes in the ejaculate of men with idiopathic infertility.
Downloads
References
2. Darenskaya M.A., Chugunova E.V., Kolesnikov S.I., Grebenkina L.A., Semenova N.V., Nikitina O.A. et al. Level of markers of oxidative damage to lipids and DNA in men with type 1 diabetes mellitus and different levels of albuminuria. Diabetes. 2022; 25(2): 120-127. doi: 10.14341/DM12765
3. Semenova N.V., Brichagina A.S., Nikitina O.A., Madaeva I.M., Kolesnikov S.I., Kolesnikova L.I. Parameters of carbonyl stress and oxidative modification of DNA during age-related menopause in women of the Russian and Buryat ethnic groups // Human Ecology. 2022; 6: 415-423. doi: 10.17816/humeco105578
4. Semenova N.V., Madaeva I.M., Brichagina A.S., Kolesnikov S.I., Kolesnikova L.I. 8-hydroxy-2'-deoxyguanosine as an oxidative stress marker in insomnia. Bulletin of Experimental Biology and Medicine. 2021; 171(3): 384-387. doi:10.1007/s10517-021-05233-0.
5. Kolesnikova L.I., Kolesnikov S.I., Kurashova N.A., Osadchuk L.V., Osadchuk A.V., Dolgikh M.I. et al. Reproductive health and peculiarities of lipid peroxidation-antioxidant defense system in men of the main ethnic groups of the Baikal region. Bulletin of Experimental Biology and Medicine. 2015; 160(1): 32-34. doi:10.1007/s10517-015-3091-6.
6. Kolesnikova L.I., Kurashova N.A., Grebenkina L.A., Labygina A.V., Suturina L.V., Dashiev B.G. and etc.Metabolic aspects of male reproductive health disorders. In the collection: Men's health. Collection of scientific papers of the VII Russian Congress with international participation. 2011: 277-278.
7. Kurashova N.A., Dashiev B.G., Kolesnikova L.I. Ethnic characteristics of antioxidant status in men with infertility. Human ecology. 2022; 10: 699-707. doi: 10.17816/humeco105642.
8. Darenskaya M.A., Rychkova L.V., Kolesnikov S.I., Semenova N.V., Kolesnikova L.I. Integral assessment of nonspecific reactions of blood lipid peroxidation in teenage girls - representatives of Siberian ethnic groups. Human ecology. 2023; 1:29-39. doi: 10.17816/humeco105586
9. Zegers-Hochschild F., Adamson G.D., Dyer S., Racowsky C., de Mouzon J., Sokol R. et al. The International Glossary on Infertility and Fertility Care, 2017. Fertil Steril. 2017; 108(3): 393-406. doi: 10.1016/j.fertnstert.2017.06.005.
10. Agarwal A., Mulgund A., Hamada A., Chyatte M.R. A unique view on male infertility around the globe. Reprod Biol Endocrinol. 2015; 13: 37. doi: 10.1186/s12958-015-0032-1.
11. Fainberg J., Kashanian J.A. Recent advances in understanding and managing male infertility. F1000Res. 2019; 8: F1000 Faculty Rev-670. doi: 10.12688/f1000research.17076.1.
12. Agarwal A., Majzoub A. Laboratory tests for oxidative stress. Indian J Urol. 2017; 33(3): 199-206. doi: 10.4103/iju.IJU_9_17.
13. Kumar N., Singh A.K. Reactive oxygen species in seminal plasma as a cause of male infertility. J Gynecol Obstet Hum Reprod. 2018; 47(10): 565-572. doi: 10.1016/j.jogoh.2018.06.008.
14. de Ligny W., Smits R.M., Mackenzie-Proctor R., Jordan V., Fleischer K., de Bruin J.P. et al. Antioxidants for male subfertility. Cochrane Database Syst Rev. 2022; 5(5): CD007411. doi: 10.1002/14651858.CD007411.pub5.
15. Smits R.M., Mackenzie-Proctor R., Yazdani A., Stankiewicz M.T., Jordan V., Showell M.G. Antioxidants for male subfertility. Cochrane Database Syst Rev. 2019; 3(3): CD007411. doi: 10.1002/14651858.CD007411.pub4.
16. Dadasheva M.N., Taranenko N.Yu., Agafonov B.V., Chudakov S.Yu. Pathogenetic therapy with the new domestic antioxidant Ethoxidol for cerebrovascular disease. Bulletin of Family Medicine. 2015;1-2: 12-16.
17. Björndahl L., Kirkman Brown J.; other Editorial Board Members of the WHO Laboratory Manual for the Examination and Processing of Human Semen. The sixth edition of the WHO Laboratory Manual for the Examination and Processing of Human Semen: ensuring quality and standardization in basic examination of human ejaculates. Fertil Steril. 2022; 117(2): 246-251. doi: 10.1016/j.fertnstert.2021.12.012.
18. Aitken R.J. Oxidative stress and the etiology of male infertility. J Assist Reprod Genet. 2016; 33(12): 1691-1692. doi:10.1007/s10815-016-0791-4.
19. Bui A.D., Sharma R., Henkel R., Agarwal A. Reactive oxygen species impact on sperm DNA and its role in male infertility. Andrologia. 2018; 50(8)
20. Wagner H., Cheng J.W., Ko E.Y. Role of reactive oxygen species in male infertility: An updated review of literature. Arab J Urol. 2017; 16(1): 35-43. doi: 10.1016/j.aju.2017.11.001.
21. Kurashova N.A., Dashiev B.G., Kolesnikov S.I., Dmitrenok P.S., Kozlovskaya E.P., Kasyanov S.P. et al. Changes in Spermatogenesis, Lipoperoxidation Processes, and Antioxidant Protection in Men with Pathozoospermia after COVID-19. The Effectiveness of Correction with a Promising Antioxidant Complex. Bull Exp Biol Med. 2022; 173(5): 606-610. doi: 10.1007/s10517-022-05596-y.
22. Kaltsas A. Oxidative Stress and Male Infertility: The Protective Role of Antioxidants. Medicina (Kaunas). 2023; 59(10): 1769. doi: 10.3390/medicina59101769.
23. Agarwal A., Cannarella R., Saleh R., Harraz A.M., Kandil H., Salvio G., et al. Impact of Antioxidant Therapy on Natural Pregnancy Outcomes and Semen Parameters in Infertile Men: A Systematic Review and Meta-Analysis of Randomized Controlled Trials. World J Mens Health. 2023; 41(1): 14-48. doi: 10.5534/wjmh.220067.
24. Łakoma K., Kukharuk O., Śliż D. The Influence of Metabolic Factors and Diet on Fertility. Nutrients. 2023; 15(5): 1180. doi: 10.3390/nu15051180.
25. Dutta S., Sengupta P., Roychoudhury S., Chakravarthi S., Wang C.W., Slama P. Antioxidant Paradox in Male Infertility: 'A Blind Eye' on Inflammation. Antioxidants (Basel). 2022; 11(1): 167. doi: 10.3390/antiox11010167.
