Resistance to acute hypoxia and changes in the phenotype and phenotypic plasticity of macrophages from mice of different genetic strains

  • S.V. Kruglov Moscow State University of Medicine and Dentistry, 20/1, Delegatskay str., Moscow, 127473, Russia; The Alexander Grigorievich and Nikolay Grogorievich Stoletov Vladimir State University , Vladimir, 87, Gorkiy str., Vladimir, 600000, Russia
  • L.Yu. Bahtina The Alexander Grigorievich and Nikolay Grogorievich Stoletov Vladimir State University , Vladimir, 87, Gorkiy str., Vladimir, 600000, Russia
  • S.V. Kalish Moscow State University of Medicine and Dentistry, 20/1, Delegatskay str., Moscow, 127473, Russia; Institute of general pathology and pathophysiology RAMS, 8, Baltiyskaya str., Moscow, 125315, Russia
  • E.V. Malysheva Moscow State University of Medicine and Dentistry, 20/1, Delegatskay str., Moscow, 127473, Russia
  • O.P. Budanova The Alexander Grigorievich and Nikolay Grogorievich Stoletov Vladimir State University , Vladimir, 87, Gorkiy str., Vladimir, 600000, Russia
  • E.B. Manukhina The Alexander Grigorievich and Nikolay Grogorievich Stoletov Vladimir State University , Vladimir, 87, Gorkiy str., Vladimir, 600000, Russia
  • N.P. Larionov Institute of general pathology and pathophysiology RAMS, 8, Baltiyskaya str., Moscow, 125315, Russia
  • I.Yu. Malyshev Moscow State University of Medicine and Dentistry, 20/1, Delegatskay str., Moscow, 127473, Russia; The Alexander Grigorievich and Nikolay Grogorievich Stoletov Vladimir State University , Vladimir, 87, Gorkiy str., Vladimir, 600000, Russia
Keywords: hypoxia, macrophages, immune response plasticity

Abstract

The aim of study was to investigate the effect of hypoxia on the macrophage phenotype and phenotypic plasticity and to determine the resistance to acute hypoxia in C57/BL mice, which have the pro-inflammatory M1 macrophage phenotype, and in BALB/c mice, which have the anti-inflammatory M2 macrophage phenotype. The following results were obtained. 1) The response of macrophages to acute hypoxia has two successive phases, the immediate, anti-inflammatory phase, and the delayed, pro-inflammatory phase. This response was more distinctly inverted in С57/BL6 M1 macrophages than in ВАLB/c M2 macrophages; 2) the effect of acute hypoxia on macrophage phenotypic plasticity depends on the genetically predetermined, original macrophage phenotype. In this process, a clear regularity was observed: hypoxia increased the capability of macrophages for changing into the pro-inflammatory M1 phenotype, while their capability for changing into the anti-inflammatory M2 phenotype remained virtually unaffected. 3) BALB/c mice were more resistant to acute hypoxia than C57/BL6 mice. Taken together, these data expand our understanding of mechanisms for pathogenetic effects of hypoxia.

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Published
2012-12-20
How to Cite
Kruglov S., Bahtina L., Kalish S., Malysheva E., Budanova O., Manukhina E., Larionov N., Malyshev I. Resistance to acute hypoxia and changes in the phenotype and phenotypic plasticity of macrophages from mice of different genetic strains // Patologicheskaya Fiziologiya i Eksperimental’naya Terapiya (Pathological physiology and experimental therapy). 2012. VOL. 56. № 3. PP. 56–61.
Issue
Vol. 56 No. 3 (2012)
Section
Original research