The activity of the Cl⁻,HCO₃⁻-activated Mg²⁺-ATPase coupled with GABAₐ-receptor of the neuronal membrane from rat brain in the presence pentylenetetrazole both in vitro and in vivo experiences

Abstract

It was found in vitro experience that GABA (0,1 µM) activated Сl^-,HCO₃^- ATPase neuronal membrane and in the presence 20 mM of the pentylenetetrazole (PTZ) the effect of the neurotransmitter on the enzyme did not appear. The pentylenetetrazole in range concentrations 15−25 M enchances the activity of the «basal» Mg²⁺-ATPase and eliminated the activating effect of Cl^-+HCO₃^- on the enzyme was found. Similar the effect of the PTZ on the activity of the «basal» Mg²⁺-ATPase activity and it is activating by anions it was observed under intraperitoneal injection of the it at dose 65 mg/kg. In particular, it was found that in the range concentration of the substrate Mg²⁺-ATP (0,5—2,5 mM) in the incubation medium the PTZ activated the «basal» Mg²⁺-ATPase and fully eliminates the activating effect of the anions on the enzyme. It was conclusion about involved of the coupled with GABA_A-receptor Cl^-,HCO₃^--activated Mg²⁺-ATPase of the neuronal membrane under synaptic transmission of the CNS and pathogenesis of the PTZ-induced convulsions of the animal.