The analysis of heteroplasmy of some subunits NADH dehydrogenase genes in homogenates of aortic intima atherosclerotic lesion

  • M.A. Sazonova The Institute for General Pathology and Pathophysiology RAMS, 8, Baltiyskaya str., Moscow, 125315, Russia, Skolkovo Innovations Centre, Institute for Atherosclerosis Research, Skolkovo Innovative Center, 143025, 100 Novaya Str., Moscow, Russia, Russian Cardiology Research Center, 15a, 3rd Cherepkovskaya str., Moscow, 121552, Russia
  • V.V. Sinyov Russian Cardiology Research Center, 15a, 3rd Cherepkovskaya str., Moscow, 121552, Russia
  • М.М. Chicheva The Institute for General Pathology and Pathophysiology RAMS, 8, Baltiyskaya str., Moscow, 125315, Russia, Russian Cardiology Research Center, 15a, 3rd Cherepkovskaya str., Moscow, 121552, Russia
  • K.Yu. Mitrofanov The Institute for General Pathology and Pathophysiology RAMS, 8, Baltiyskaya str., Moscow, 125315, Russia, Russian Cardiology Research Center, 15a, 3rd Cherepkovskaya str., Moscow, 121552, Russia
  • A.V. Zhelankin The Institute for General Pathology and Pathophysiology RAMS, 8, Baltiyskaya str., Moscow, 125315, Russia, Russian Cardiology Research Center, 15a, 3rd Cherepkovskaya str., Moscow, 121552, Russia
  • A.N. Orekhov The Institute for General Pathology and Pathophysiology RAMS, 8, Baltiyskaya str., Moscow, 125315, Russia, Russian Cardiology Research Center, 15a, 3rd Cherepkovskaya str., Moscow, 121552, Russia
  • I.A. Sobenin The Institute for General Pathology and Pathophysiology RAMS, 8, Baltiyskaya str., Moscow, 125315, Russia, Skolkovo Innovations Centre, Institute for Atherosclerosis Research, Skolkovo Innovative Center, 143025, 100 Novaya Str., Moscow, Russia
Keywords: mitochondrial mutation, homogenate, intima, aorta, heteroplasmy, atherosclerosis, lipofibrous plaque, gene, NADH dehydrogenase

Abstract

A comparative analysis of heteromlasmy level of four mutations in mitochondrial genes of subunits 1, 2, 5 and 6 NADH dehydrogenase in DNA samples was carried out. The DNA samples were isolated from total homogenates of intima of 10 aortas affected with atherosclerosis and normal ones. It was found that the heteroplasmy level of mutations G14459A and C5178A in homogenates with atherosclerotic lesions is significantly higher compared to total homogenates from normal intima in the investigated aortas.

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Published
2012-12-20
How to Cite
Sazonova M., Sinyov V., Chicheva М., Mitrofanov K., Zhelankin A., Orekhov A., Sobenin I. The analysis of heteroplasmy of some subunits NADH dehydrogenase genes in homogenates of aortic intima atherosclerotic lesion // Patologicheskaya Fiziologiya i Eksperimental’naya Terapiya (Pathological physiology and experimental therapy). 2012. VOL. 56. № 4. PP. 71–74.
Issue
Vol. 56 No. 4 (2012)
Section
Original research