The association of heteroplasmy of mutations in mitochondrial RNA genes with homogenates of intima aorta affected with atherosclerosis

  • M.A. Sazonova The Institute for General Pathology and Pathophysiology RAMS, 8, Baltiyskaya str., Moscow, 125315, Russia, Skolkovo Innovations Centre, Institute for Atherosclerosis Research, Skolkovo Innovative Center, 143025, 100, Novaya Str., Moscow, Russia, Russian Cardiology Research Center, 15a, 3rd Cherepkovskaya str., Moscow, 121552, Russia
  • V.V. Sinyov Russian Cardiology Research Center, 15a, 3rd Cherepkovskaya str., Moscow, 121552, Russia
  • М.М. Chicheva The Institute for General Pathology and Pathophysiology RAMS, 8, Baltiyskaya str., Moscow, 125315, Russia, Russian Cardiology Research Center, 15a, 3rd Cherepkovskaya str., Moscow, 121552, Russia
  • K.Yu. Mitrofanov The Institute for General Pathology and Pathophysiology RAMS, 8, Baltiyskaya str., Moscow, 125315, Russia, Russian Cardiology Research Center, 15a, 3rd Cherepkovskaya str., Moscow, 121552, Russia
  • A.V. Zhelankin The Institute for General Pathology and Pathophysiology RAMS, 8, Baltiyskaya str., Moscow, 125315, Russia, Russian Cardiology Research Center, 15a, 3rd Cherepkovskaya str., Moscow, 121552, Russia
  • A.N. Orekhov The Institute for General Pathology and Pathophysiology RAMS, 8, Baltiyskaya str., Moscow, 125315, Russia, Russian Cardiology Research Center, 15a, 3rd Cherepkovskaya str., Moscow, 121552, Russia
  • I.A. Sobenin The Institute for General Pathology and Pathophysiology RAMS, 8, Baltiyskaya str., Moscow, 125315, Russia, Skolkovo Innovations Centre, Institute for Atherosclerosis Research, Skolkovo Innovative Center, 143025, 100, Novaya Str., Moscow, Russia, Russian Cardiology Research Center, 15a, 3rd Cherepkovskaya str., Moscow, 121552, Russia
Keywords: mitochondrial mutation, homogenate, intima, aorta, heteroplasmy, atherosclerosis, lipofibrous plaque, gene, RNA

Abstract

In this paper a comparative analysis of the heteroplasmy level of 4 mitochondrial genome mutations in DNA samples isolated from total homogenates of 10 normal and affected with atherosclerosis intima of aortas of people, who died in a result of an accident or a sudden death, is represented. It was found that the heteroplasmy level of the mutant allele A1555G and G12315A in homogenates of atherosclerotic lesions is significantly higher compared to total homogenates from normal intima of in the investigated aortas.

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Published
2012-12-20
How to Cite
Sazonova M., Sinyov V., Chicheva М., Mitrofanov K., Zhelankin A., Orekhov A., Sobenin I. The association of heteroplasmy of mutations in mitochondrial RNA genes with homogenates of intima aorta affected with atherosclerosis // Patologicheskaya Fiziologiya i Eksperimental’naya Terapiya (Pathological physiology and experimental therapy). 2012. VOL. 56. № 4. PP. 67–70.
Issue
Vol. 56 No. 4 (2012)
Section
Original research