Effects of car-regimen autohemochemotherapy on redox-dependent processes in erythrocytes of tumor-bearing organism in experimental ovarian cancer

Abstract

The purpose of the study was to investigate the effect of the scheme autohemochemotherapy ATS on redox-dependent processes in red blood cells of tumor-bearing organism at an experimental ovarian cancer. Methods. We studied the dynamics of redox-dependent processes in red blood cells of rats with experimental ascitic ovarian tumor during CAP-regimen autohemochemotherapy (cyclophosphamide, doxorubicin and cisplatin). We assessed the indicators of oxidative modification of proteins in erythrocytes – carbonyl derivatives at λ = 346 nm, 370 nm, 430 nm and 530 nm, the parameters of lipid peroxidation – malondialdehyde, ketodienes, diene conjugates, Schiff bases; the enzymatic part of antioxidant system – the activity of catalase, glutathione transferase and superoxide dismutase biochemically. Results. The red blood cells of tumor-bearing animals were found having increased the products of lipid peroxidation and oxidative modification of proteins while reducing the activity of antioxidant enzymes, suggesting a state of oxidative and carbonyl stress. Conclusion. We showed that extracorporeal incubation of cytotoxic drugs used in the CAP scheme with autoblood prior to infusion – the method of autohemochemotherapy – either in monochemotherapy, or in CAP-regimen, decreases the levels of lipid peroxidation, oxidative modification of proteins and increases activity of first line antioxidant defense enzymes – catalase and superoxide dismutase in circulating red blood cells. Such dynamics of redox-dependent processes suggests a stabilizing effect of autohemochemotherapy on circulating erythrocytes in a tumor-bearing organism.