The prognostic value of determining gamma-glutamyltranspeptidase activity in blood plasma of rats under the action of electromagnetic radiation

Abstract

The aim of the research was to measure the activity of γ-glutamyltranspeptidase in rat plasma under the action of decimeter-range electromagnetic radiation and to assess correlations between the enzyme activity and major indexes of the antioxidant defense. Methods. Experiments were performed on rats exposed to electromagnetic radiation for 3 mos. The γ-glutamyltranspeptidase activity was measured in blood plasma spectrophotometrically at 1, 2, and 3 mos. Correlations were determined between the γ-glutamyltranspeptidase activity and the concentration of reduced glutathione and between the γ-glutamyltranspeptidase activity and the concentration of ceruloplasmin in plasma. Results. The long-term exposure of animals to decimeter-range electromagnetic radiation resulted in increases in the γ-glutamyltranspeptidase activity and concentrations of ceruloplasmin and glutathione. Significant correlations were found between the γ-glutamyltranspeptidase activity and the red cell concentration of reduced glutathione and between the γ-glutamyltranspeptidase activity and ceruloplasmin concentration. Conclusion. An experimental study of the effect of electromagnetic radiation on the activity of γ-glutamyltranspeptidase in rat plasma was performed. The enzyme γ-glutamyltranspeptidase along with glutathione and ceruloplasmin, are targets for the action of electromagnetic radiation. Electromagnetic radiation is a possible factor in the development of cardiovascular pathology.