Inhibition of tumor growth in osteosarcoma cell culture with mir162a miicroRNA

  • Dmitry Ju. Grebnev Ural State Medical University, Repina St. 3, Yekaterinburg, 620028, Russian Federation https://orcid.org/0000-0002-5698-8404
  • I.Ju. Maklakova Ural State Medical University, Repina St. 3, Yekaterinburg, 620028, Russian Federation https://orcid.org/0000-0002-6895-7947
  • D.O. Kornilov Ural State Medical University, Repina St. 3, Yekaterinburg, 620028, Russian Federation https://orcid.org/0000-0001-5311-1247
  • V.M. Simarzina Ural State Medical University, Repina St. 3, Yekaterinburg, 620028, Russian Federation
  • M.A. Tryapitsyn Ural State Medical University, Repina St. 3, Yekaterinburg, 620028, Russian Federation
  • Ju.A. Kazancev Ural State Medical University, Repina St. 3, Yekaterinburg, 620028, Russian Federation
  • D.L. Zornikov Ural State Medical University, Repina St. 3, Yekaterinburg, 620028, Russian Federation https://orcid.org/0000-0001-9132-215X
  • Ju.V. Grigoryeva Ural State Medical University, Repina St. 3, Yekaterinburg, 620028, Russian Federation
  • F.A. Fadeev Ural State Medical University, Repina St. 3, Yekaterinburg, 620028, Russian Federation; Institute of Medical Cell Technologies, Karla Marxa St. 22a, Yekaterinburg, 620026, Russian Federation
DOI: https://doi.org/10.25557/0031-2991.2023.01.48-55
Keywords: MTOR; mir162a; SAOS-2, microRNA; RNA interference; osteosarcoma; apoptosis; necrosis; viability; autophagy

Abstract

Aim. To study the effect of mir162a microRNA on the viability of SAOS-2 osteosarcoma tumor cells. Methods. The study was performed on SAOS-2 cell culture. The mir162a microRNA was transfected at a dose of 90 pmol. At 24, 48, 72 hours, viable, apoptotic, and necrotic cells were counted by fluorescence microscopy in the transfected cell culture. Also, the level of cell autophagy was evaluated. Results. The transfection of SAOS-2 with mir162a decreased the proportion of viable cells and increased the proportion of apoptotic and necrotic cells at 24-72 hours after the procedure. Compared to the negative control, the proportion of viable cells in the mir162a-transfected SAOS-2 samples was decreased by 29.3%, 33.9%, 47.4% at 24, 48, and 72 hours, respectively. The transfection caused a 75% decrease in the autophagy level. Conclusion. The results suggest that mir162a microRNA has a therapeutic potential for the pathogenetic therapy of malignant neoplasms.

Downloads

Download data is not yet available.
Published
2023-03-17
How to Cite
Grebnev D. J., Maklakova I., Kornilov D., Simarzina V., Tryapitsyn M., Kazancev J., Zornikov D., Grigoryeva J., Fadeev F. Inhibition of tumor growth in osteosarcoma cell culture with mir162a miicroRNA // Patologicheskaya Fiziologiya i Eksperimental’naya Terapiya (Pathological physiology and experimental therapy). 2023. VOL. 67. № 1. PP. 48–55.
Issue
Vol. 67 No. 1 (2023)
Section
Original research