Comparison of synergistic anticoagulant and fibrin depolymerizing properties of heparinoid extracts from hybrid varieties of peonies («Kuindzhi», «Academician Sadovnichy», «Coral») and tree peony P. suffruticosa

Abstract

At present, developing safe drugs to combat thrombotic complications arising from various functional (cardiovascular, pulmonary, etc.) and viral (Covid-19) diseases is an acute problem. Usually, heparin-like anticoagulants are used in treatment of these diseases. These substances were found in tree and herbaceous peonies. The aim of this investigation was to compare the anticoagulant action and processes of fibrin depolymerization of hybrid peonies («Kuindzhi», «Academician Sadovnichy» and «Coral») and tree peonies (P. suffruticosa) that are used for heparin production. Roots of hybrid peonies («Kuindzhi», «Academician Sadovnichiy», «Coral») and P. suffruticosa were the object of research. Methods. Procedures to prepare peony root extractions were developed, and the nature of the active agent was determined. The anticoagulant, fibrinolytic activity, and the degree of fibrin polymerization were determined after addition to rat plasma. Results. For the first time, heparin-like substances were detected in extracts from hybrid peonies. At concentrations of 1 and 10^-3 mg/ml, extracts from all hybrid peonies exhibited anticoagulant and fibrin-depolymerizing activity (FDPA), but to different degrees. Anticoagulant activity (ACTV) increased by 32-48%, thrombin time (TT) by 8-15%, prothrombin time by 14-21%, total fibrinolytic activity (TFA) by 27-50%, and FDPA by 49-80% compared to control. These activities were less in tree peony extract: ACTV increased by 23%, TT by 7%, TFA by 18-20%, and FDPA by 47% compared to control. Conclusion. For the first time the ability of hybrid peony root extract to show synergistic, anticoagulant and fibrin depolymerization effects exceeding those of extract from tree peony was established. These data demonstrate that hybrid peonies should be studied as sources of antithrombotic and anti-atherosclerotic agents.