Effect of damage integrity rat brain synaptic membranes on the functional activity GABAₐ-receptor/Cl⁻-ionophore complex in the CNC
Abstract
Functional activity of the GABAA-receptor/Cl-ionophore complex was investigated the muscimol-stimulated entry of the radioactive isotope 36Cl− in synaptoneurosomes in changing the structure and permeability of neuronal membranes. Integrity of the membranes was damaged by removal of Ca+2 and Mg+2 from the incubation medium and by the method of freezing-thawing synaptoneurosomes. In both cases, an increase in basal 36Cl− entry into synaptoneurosomes, indicating increased nonspecific permeability of neuronal membranes, and decreased activity the GABAA-receptor/Cl−- ionophore complex. The conclusion about the relationship of processes damage neuronal membranes and reducing the inhibitory processes in the epileptic focus.