Immune modulating activity of forsythoside b at experimental immune supression

Abstract

The purpose of the study is to determine the immune modulating activity of phenylpropanoid (forsythoside B, ForB) isolated from the tubers of Phlomoides tuberosa (L.) Moench (Lamiaceae) on the cellular and humoral immune response at the azathioprine immune suppression. Methods. Experiments were carried out on CBA male mice (n=100, 18‒20 g). Immune deficiency was modeled by the intragastrical introduction of azathioprine in the dose 50 mg/kg once a day for 5 days. The experimental group of animals administered ForB once a day intragastrically in dose 1 mg/kg for 14 days against the background of azathioprine immune suppression. The intact group administered the purified water according to the analogous scheme. The activity of the tested compound on the cellular immunity was evaluated in the reaction of delayed-type hypersensitivity (DTH) according to the standard method of local DTH. Condition of the humoral immunity was estimated by the number of antibody-forming cells (AFC) determined by the method of local hemolysis by A.J. Cunningham (1965). Statistical analysis was performed using standard methods of variation statistics using parametric Student’s t-test. Results. It was established that the administration of the ForB to animals significantly reduced suppressive effect of cytostatic azathioprine on the DTH reaction and antibody response. The use of the tested compound was resulted to the increase of the DTH-index (1.5 times) and the value of absolute and relative numbers of AFC in 1.3 and 1.9 times comparing with the control group. Conclusion. The results obtained allow to conclude that the ForB showed the marked immune modulating activity at the azathioprine-induced immune suppression demonstrating its perceptiveness as a new and effective immune modulators.