MicroRNAs and polymorphism of genes of biogenesis in the pathogenesis of atherosclerosis

Abstract

The purpose — to determine the condition expression of pro-angiogenic and anti-angiogenic microRNAs and specifics of gene polymorphisms their biogenesis in the severe atherosclerosis and to introduce the pathophysiologic analysis of the significance of the data. Methods. The study included 30 men suffering from essential hypertension, 20 men who have the diagnosis of cardiosclerosis and 15 healthy men. Proangiogenic miR-126 and miR-155, angiogenic miR-221 and miR-222 were defined in the blood plasma for each patient. During genotyping single nucleotide polymorphisms of genes involved in the biogenesis microRNAs, such as AGO1 (A/G), DGCR8 (G/A), GEMIN4 (A/G), DROSHA (A/C) were determined. The analysis of the association of changes in the expression of microRNAs and polymorphisms of genes with the incidence of essential hypertension and cardiosclerosis was carry out. Results. We found the association between genotype AA GEMIN4 gene and the risk of essential hypertension. In the study of microRNAs expression levels of proangiogenic miR-126 and antiangiogenic miR-221, miR-222 in patients with essential hypertension and cardiosclerosis were significantly higher than in the control group. While the level of expression of proangiogenic miR-155 in the blood plasma remains unchanged in both groups. The study of integral portraits of covariance matrices and variance miRNAs led to the conclusion that in the control group of healthy people the dominant variation role belongs miR-126 and in the groups of patients with essential hypertension and cardiosclerosis — miR-221. Conclusion. The results indicate that the quantitative and qualitative evaluation of the studied miR-126, miR-155, miR-221, miR-222 in patients with essential hypertension and cardiosclerosis confirms the correlation of these microRNAs with the studied diseases of the cardiovascular system.