Influence of partial hepatectomie on ammoniumdetoxications function of liver at chronic tetrachlorcarbon hepatitis

Abstract

The purpose. To study the effect of partial hepatectomy (PH) on the main ways of ammonia detoxication in the liver (synthesis of urea and glutamine) in chronic tetrachlorcarbon (CCl 4 ) hepatitis. Methods. The experiments were performed on 165 white outbred rats (females) weighing 180—220 g Chronic CCl 4 -hepatitis was reproduced by subcutaneous injection of 50% CCl 4 solution in olive oil (0.1 ml/100g of body weight,65 days, through the day with two two-week breaks between 6—7 and 13—14 injections). PH conducted electrocautery, removing part of the left lobe of the liver (15—20% by weight of the body) to 65th (and last) day of the introduction of the CCl 4 . Animals were studied after 65 days of development of CCl4-hepatitis on day 3, 7 and 14 days after laparotomy («falsely operated» animals) and partial hepatectomy. In subcellular fractions of the liver investigated the activity of phosphatdependent glutaminase (FDG), glutamatdehydrogenaze (GDG), glutaminsintetaze (GS), arginase. In the liver tissue investigated the content of ammonia, glutamine, glutamate and urea. Results. Found that on 65-th day of the development of CCl 4 in the liver decreases the concentration of ammonia, glutamine, glutamate, urea, and activity of GS, GDG and arginase. Activity FDG was not changed. The use of PH on the background of chronic CCl 4 -hepatitis has a short-term (3 days) a stimulating effect on the activity FDG, GS, postponed (14 days) the inhibitory effect on the activity of GDG. This was accompanied by an increase in the concentration in the liver of ammonia within 14 days of the postoperative period on the background of maintaining reduced concentrations of glutamine and glutamate it. The stimulatory effect of CHA on the activity of arginase is saved to the 14th day of the postoperative period, however, the concentration of urea in the liver remained below normal. Conclusions. The obtained results show that CGA on the background of chronic hepatitis increases the pathological impact of CCl4 on amniocentesis liver function.