Reprogrammed M1 macrophages with inhibited STAT3, STAT6 and/or SMAD3 extends lifespan of mice with experimental carcinoma

  • S. V. Kalish Moscow State University of Medicine and Dentistry n.a. A.I. Evdokimov
  • S. V. Lyamina Moscow State University of Medicine and Dentistry n.a. A.I. Evdokimov
  • A. A. Raetskaya Moscow State University of Medicine and Dentistry n.a. A.I. Evdokimov
  • I. Yu. Malyshev Moscow State University of Medicine and Dentistry n.a. A.I. Evdokimov; Institute of General Pathology and Pathophysiology
DOI: https://doi.org/10.25557/0031-2991.2017.02.4-9
Keywords: Keywords: macrophages; Ehrlich carcinoma; tumor microenvironment; reprogramming; cytokines.

Abstract

Objective. Reprogramming of M1 macrophage phenotype with inhibited M2 phenotype transcription factors, such as STAT3, STAT6 and SMAD and assess their impact on the development of Ehrlich carcinoma (EC) in vitro and in vivo. Methods. Tumor growth in vitro was initiated by addition of EC cells in RPMI-1640 culture medium and in vivo by intraperitoneal of EC cell injection into mice. Results. It was found that M1 -STAT3/6- SMAD3 macrophages have a pronounced anti-tumor effect in vitro, and in vivo, which was greater than anti-tumor effects of M1, M1 -STAT 3/6 , M1 -SMAD3 macrophages and cisplatin. Conclusion. M1 macrophages with inhibited STAT3, STAT6 and/or SMAD3 effectively restrict tumor growth. The findings justify the development of new anti-tumor cell therapy technology.

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Author Biographies

S. V. Kalish, Moscow State University of Medicine and Dentistry n.a. A.I. Evdokimov

Kalish Sergey Valerevich — senior laboratory assistant of the Department of Pathological Physiology of the State Pedagogical University of the Moscow State University of Medicine and Dentistry n.a. A.I. Evdokimov. 20/1 Delegatskaya Str., Moscow, 127473, Russian Federation; e-mail: anahoretes@mail.ru

S. V. Lyamina, Moscow State University of Medicine and Dentistry n.a. A.I. Evdokimov

Lyamina S.V. — Doctor of Medical Sciences, Professor of the Department of Pathological Physiology of Moscow State University of Medicine and Dentistry n.a. A.I. Evdokimov. 20/1 Delegatskaya, Moscow, 127473, Russian Federation; e-mail: svlvs@mail.ru

A. A. Raetskaya, Moscow State University of Medicine and Dentistry n.a. A.I. Evdokimov

Raetskaya A.A. — Laboratory Assistant of the Department of Pathological Physiology of Moscow State University of Medicine and Dentistry n.a. A.I. Evdokimov. 20/1 Delegatskaya, Moscow, 127473, Russian Federation; e-mail: mohnush@mail.ru

I. Yu. Malyshev, Moscow State University of Medicine and Dentistry n.a. A.I. Evdokimov; Institute of General Pathology and Pathophysiology

Malyshev, Igor Y., Doctor of Medical Sciences, Professor, Head of the Department of Pathological Physiology, Moscow State University of Medicine and Dentistry n.a. A.I. Evdokimov of the Ministry of Health of Russian Federation. 20/1 Delegatskaya, Moscow, 127473, Russian Federation, Head of the Laboratory of Stress and Adaptation of the Institute of General Pathology and Pathophysiology Russian Academy of Sciense. 8 Baltiyskaya Str., Moscow, 125315, Russian Federation; e-mail: iymalyshev1@gmail.com

Published
2017-07-13
How to Cite
Kalish S. V., Lyamina S. V., Raetskaya A. A., Malyshev I. Y. Reprogrammed M1 macrophages with inhibited STAT3, STAT6 and/or SMAD3 extends lifespan of mice with experimental carcinoma // Patologicheskaya Fiziologiya i Eksperimental’naya Terapiya (Pathological physiology and experimental therapy). 2017. VOL. 61. № 2. PP. 4–9.
Issue
Vol. 61 No. 2 (2017)
Section
Original research