Oxidation-antioxidation system in hemorrhagic shock and infusion therapy with regulators of the synthesis of nitric oxide in experiment

Abstract

Different types of shock, including hemorrhagic shock, are associated with oxidative stress. The oxidant-antioxidant system plays a major role during severe blood loss. The aim of these experiments was to study circulation, oxygen regimen and oxidant-antioxidant systems in hemorrhagic shock and infusion therapy. Methods. Experiments were performed on thiopental-anesthetized rabbits. Hemorrhagic shock was induced by blood loss of 18.5±0.6 ml/kg body weight to reduce blood pressure to 40–60 mm Hg for 30-40 min. Then infusion of a volume expander was started. Effectiveness of the infusion therapy was evaluated by indices of systemic hemodynamics, microcirculation, and the oxidant-antioxidant system. The infusion solution consisting of isotonic sodium chloride was supplemented with a nitric oxide (NO) donor, Oxacom® (0.7 µmol/kg), and a NO precursor, L-arginine (200 mg/kg). Results. Blood loss resulted in considerable changes in systemic hemodynamic, microcirculation, metabolism, and oxygen balance. The content of peroxidation products directly depended on the degree of circulatory disorders. Hemodynamic indices were closely correlated with blood levels of malonic dialdehyde and ceruloplasmin. Oxacom® and L-arginine exerted a beneficial effect on circulation and the oxidant-antioxidant system. Conclusion. NO-enhanced infusion therapy increased animal survival. In control experiments (infusion of isotonic sodium chloride), the survival rate was 42% while the sodium chloride solution supplemented with Oxacom® and L-arginine increased the survival rate to 77% and 59%, respectively.