Comparative analysis of hematopoietic activity of total RNA from bone marrow cells and splenocytes of rats with chronic benzene-induced anemia

Abstract

Aim. To evaluate therapeutic properties of total RNA on a model of chronic hypoplastic anemia and to elucidate features of the hemopoietic effect of total RNA isolated from bone marrow splenic lymphoid cells. Methods. Experiments were performed on 36 white mongrel rats weighing 230–250 g. Chronic toxic anemia was induced by subcutaneous injections of a mixture of chemically pure benzene (0.05 ml/100 g) and sterile vegetable oil. The mixture was injected three times with an interval of 7 days. Control animals (group 1 in each series) received intravenous injections of an equal volume of saline at the same time intervals. Two experimental series were performed. In series 1 and 2, hemopoietic activity of total RNAs from splenic lymphoid cells and from bone marrow, respectively, was evaluated. Total RNA was obtained from splenic lymphoid cells and from bone marrow of intact rats and rats subjected to hemorrhage (2% of body weight) 17 h prior to the RNA isolation. Total RNA was isolated by guanidine-thiocyanate-phenol-chloroform extraction. The effect of total RNA on the erythroid lineage in the bone marrow was evaluated by the quantitative and qualitative composition of erythroid islets in Pappenheim-stained preparations. Results. Comparing the hematopoietic activity of total RNA isolated from bone marrow and splenic lymphoid cells of healthy animals and administered to rats with experimental chronic benzene anemia showed that the difference in cell composition of the lymphoid organs, sources of the RNAs, impacted on the RNA hemopoietic properties. The total RNA from splenic lymphoid cells of intact animals contributed primarily to the recovery of leukocyte count while the RNA from the bone marrow of intact rats did not significantly influence leukopoiesis during the early regeneration period. It was also shown that the total RNA extracted from the bone marrow generally provided a more intensive hemopoiesis. In addition, the erythropoietic activity of total RNA isolated from the cells of both lymphoid organs of the donor rats stimulated by blood loss was higher than that of the total RNA isolated from intact rats. Conclusion. The total RNA isolated from bone marrow and lymphoid cells spleen exerted a hemopoietic effect in hypoplastic anemia. The erythropoietic activity of total RNA from both lymphoid organs of animals after hemorrhage was higher than the RNA leukopoietic activity.