Diagnostic capabilities of the electrophoretic mobility of red blood cells and buccal cells in stress

  • A. V. Deryugina Department of Physiology and Anatomy, Institute of Biology and Medicine, the N.I. Lobachevsky National Research State University of Nizhniy Novgorod, Prospekt Gagarina 23, Nizhniy Novgorod 603950, Russian Federation
  • Marina N. Ivashchenko State Agricultural Academy of Nizhniy Novgorod, Prospekt Gagarina 97, Nizhniy Novgorod 603107, Russian Federation
  • P. S. Ignatyev Joint-Stock Company Production Association E.S. Yamalov Ural Optical and Mechanical Plant, Vostochnayay Str. 33B, Ekaterinburg, Russian Federation
  • A. G. Samodelkin State Agricultural Academy of Nizhniy Novgorod, Prospekt Gagarina 97, Nizhniy Novgorod 603107, Russian Federation
  • M. V. Zolotova Department of Physiology and Anatomy, Institute of Biology and Medicine, the N.I. Lobachevsky National Research State University of Nizhniy Novgorod, Prospekt Gagarina 23, Nizhniy Novgorod 603950, Russian Federation
  • M. A. Shabalin Department of Physiology and Anatomy, Institute of Biology and Medicine, the N.I. Lobachevsky National Research State University of Nizhniy Novgorod, Prospekt Gagarina 23, Nizhniy Novgorod 603950, Russian Federation
  • E. Gracheva Department of Physiology and Anatomy, Institute of Biology and Medicine, the N.I. Lobachevsky National Research State University of Nizhniy Novgorod, Prospekt Gagarina 23, Nizhniy Novgorod 603950, Russian Federation
DOI: https://doi.org/10.25557/0031-2991.2019.01.106-111
Keywords: RBC electrophoretic mobility, buccal cells, stress

Abstract

Diagnostics of stress reaction using an analysis of hormonal state is a difficult, costly, and labor-consuming research. Therefore, searching for information technologies, which could be easily used in any medical laboratory and would allow evaluating the stress reaction is relevant. We studied the electrophoretic mobility of red blood cells (RBC) and buccal epithelial cells of rats exposed to stress induced by a single or repeated intraperitoneal epinephrine injection. This study showed that these methods could be used as the marker characteristics of stress reaction intensity and genotoxicity. Qualitative changes in the electrophoretic mobility of RBC were similar but quantitative characteristics depended on the acting force. Under a weak stimulus, changes in the electrophoretic mobility of RBC were slight and associated with development of adaptive processes related with repair of genetic damage. A further increase in the acting force led to a sharp change in the electrophoretic mobility of RBC and an increase in the number of epithelial cells with pathological alterations.

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Published
2019-03-11
How to Cite
Deryugina A. V., Ivashchenko M. N., Ignatyev P. S., Samodelkin A. G., Zolotova M. V., Shabalin M. A., Gracheva E. . Diagnostic capabilities of the electrophoretic mobility of red blood cells and buccal cells in stress // Patologicheskaya Fiziologiya i Eksperimental’naya Terapiya (Pathological physiology and experimental therapy). 2019. VOL. 63. № 1. PP. 106–111.
Issue
Vol. 63 No. 1 (2019)
Section
Methods