The role of cholemia in development of cardiodepression after subtotal liver resection in rats

Abstract

Aim. To assess the role of cholemia in development of myocardial dysfunction following the subtotal hepatic resection in rats. Methodology. Experiments were conducted on 48 white mongrel male rats. Subtotal atypical 80% liver resection was performed on 38 animals using an original method; the control group consisted of 10 intact animals. Total serum concentration of bile acids was measured using the enzymatic colorimetric method in intact animals and on days 1, 3, and 7 after the liver resection. Myocardial contractile function and metabolism were directly evaluated on spontaneously contracting isolated hearts of intact animals. During the heart perfusion with added bile, a set of contractile force and velocity indices was calculated; glucose and lactate concentrations and aspartate aminotransferase activity were measured in the coronary perfusate. Results. The total serum content of bile acids was increased in operated animals at all study points. When bile was added to the perfusion solution, the isolated hearts developed bradycardia, decreases in force and speed indices of the contractile function, increased glucose consumption, and release of lactate and aspartate aminotransferase into the coronary perfusate. Conclusion. The study showed the importance of cholemia following the subtotal liver resection for the development of functional and metabolic disorders of the myocardium.