Some features of targeted proteomics based on the use of HPLC-MS/MS with the registration of selective transitions

  • Edward D. Virus The Institute of General Pathology and Pathophysiology 8, Baltiyskaya st., Moscow, 125315
  • A. V. Ivanov The Institute of General Pathology and Pathophysiology 8, Baltiyskaya st., Moscow, 125315
  • P. O. Bulgakova The Institute of General Pathology and Pathophysiology 8, Baltiyskaya st., Moscow, 125315
  • A. A. Kubatiev The Institute of General Pathology and Pathophysiology 8, Baltiyskaya st., Moscow, 125315
DOI: https://doi.org/10.25557/0031-2991.2018.04.219-222
Keywords: targeted proteomics, HPLC-MS/MS, SRM

Abstract

This article introduces the reader to some methodological aspects of an experiment using HPLC-MS/MS with the registration of selective transitions in the targeted proteomics. Special attention is paid to the selection criterion of a peptide used as a surrogate analyte of the protein to be determined. The approaches to the selection of selective transitions for the selective detection of peptides are described in detail.

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Published
2018-11-21
How to Cite
Virus E. D., Ivanov A. V., Bulgakova P. O., Kubatiev A. A. Some features of targeted proteomics based on the use of HPLC-MS/MS with the registration of selective transitions // Patologicheskaya Fiziologiya i Eksperimental’naya Terapiya (Pathological physiology and experimental therapy). 2018. VOL. 62. № 4. PP. 219–222.
Issue
Vol. 62 No. 4 (2018)
Section
Methods