Some features of targeted proteomics based on the use of HPLC-MS/MS with the registration of selective transitions

  • Edward D. Virus The Institute of General Pathology and Pathophysiology 8, Baltiyskaya st., Moscow, 125315
  • A. V. Ivanov The Institute of General Pathology and Pathophysiology 8, Baltiyskaya st., Moscow, 125315
  • P. O. Bulgakova The Institute of General Pathology and Pathophysiology 8, Baltiyskaya st., Moscow, 125315
  • A. A. Kubatiev The Institute of General Pathology and Pathophysiology 8, Baltiyskaya st., Moscow, 125315
DOI: https://doi.org/10.25557/0031-2991.2018.04.219-222
Keywords: targeted proteomics, HPLC-MS/MS, SRM

Abstract

This article introduces the reader to some methodological aspects of an experiment using HPLC-MS/MS with the registration of selective transitions in the targeted proteomics. Special attention is paid to the selection criterion of a peptide used as a surrogate analyte of the protein to be determined. The approaches to the selection of selective transitions for the selective detection of peptides are described in detail.

Downloads

Download data is not yet available.
Published
21-11-2018
How to Cite
Virus E. D., Ivanov A. V., Bulgakova P. O., Kubatiev A. A. Some features of targeted proteomics based on the use of HPLC-MS/MS with the registration of selective transitions // Patologicheskaya Fiziologiya i Eksperimental’naya Terapiya (Pathological physiology and experimental therapy). 2018. VOL. 62. № 4. PP. 219–222.
Issue
Vol. 62 No. 4 (2018)
Section
Methods