Effect of anaferon for children on the in vitro life cycle of influenza virus A/H1N1

Abstract

The aim of this study was to evaluate a possible direct effect of Anaferon for Children on the life cycle of influenza A virus during infection development and a dose response of the antiviral effect in vitro. Methods. The in vitro antiviral activity of Anaferon for Children was studied on cultured MDCK cells and reference strains of influenza virus A (H1N1) pdm09: A/California/07/09 and A/California/04/09, both from the WHO. Inhibition of viral replication by Anaferon for Children and its effect on ultrastructural features of the influenza morphogenesis were evaluated using electron microscopy. Results. The study demonstrated a dose dependence of Anaferon for Children antiviral activity for three dilutions — 1/8, 1/12, and 1/16. Anaferon for Children affected the process of virion budding as compared to placebo. Conclusion. The study showed that the anti-influenza action of Anaferon for Children was dose-dependent and confirmed that this drug was effective against two strains of pandemic A/H1N1 influenza. Furthermore, Anaferon for children disrupted one or several stages of the virus life cycle.

                                                                                                                                                                        Conflict of interest. OOO «NPF «Materia Medica Holding» was sponsor of the described studies. Nikiforova M.V., Gorbunov E.A. and Tarasov S.A. are employees of OOO «NPF «Materia Medica Holding». OOO «NPF «Materia Medica Holding» produces and markets drugs «Anaferon» and «Anaferon for children». The article’s materials have not been published anywhere before. Fundation. The study was sponsored by OOO «NPF «Materia Medica Holding».