Some features of targeted proteomics based on the use of HPLC-MS/MS with the registration of selective transitions

Authors

  • Edward D. Virus The Institute of General Pathology and Pathophysiology 8, Baltiyskaya st., Moscow, 125315
  • A. V. Ivanov The Institute of General Pathology and Pathophysiology 8, Baltiyskaya st., Moscow, 125315
  • P. O. Bulgakova The Institute of General Pathology and Pathophysiology 8, Baltiyskaya st., Moscow, 125315
  • A. A. Kubatiev The Institute of General Pathology and Pathophysiology 8, Baltiyskaya st., Moscow, 125315

DOI:

https://doi.org/10.25557/0031-2991.2018.04.219-222

Keywords:

targeted proteomics, HPLC-MS/MS, SRM

Abstract

This article introduces the reader to some methodological aspects of an experiment using HPLC-MS/MS with the registration of selective transitions in the targeted proteomics. Special attention is paid to the selection criterion of a peptide used as a surrogate analyte of the protein to be determined. The approaches to the selection of selective transitions for the selective detection of peptides are described in detail.

Downloads

Download data is not yet available.

Published

21-11-2018

Issue

Vol. 62 No. 4 (2018)

Section

Methods

How to Cite

Virus E. D. [et al.]. Some features of targeted proteomics based on the use of HPLC-MS/MS with the registration of selective transitions // Patologicheskaya Fiziologiya i Eksperimental’naya Terapiya (Pathological physiology and experimental therapy). 2018. VOL. 62. № 4. PP. 219–222.

Similar Articles

1-10 of 30

You may also start an advanced similarity search for this article.

Most read articles by the same author(s)