The influence of nanosilver solution on nonspecific proteolysis processes in gastric mucosa in prophylactic and treatment of experimental gastric ulcer

Abstract

The purpose: studying the effects of nanosilver solution in prophylactic and therapeutic oral application in the simulation of acute gastric ulcers based on assessment of reactions of non-specific proteases and their inhibitors at a systemic and local level. Methods. The experimental research was carried out on 49 white male rats of “Wistar” line. In the first series of experiments, nanosilver solution at the concentration of 0.01 g/l was given to drink to the rats of the first group (n=10) during 21 days, distilled water - in the second group (n=10). After 21 days in both groups was performed simulation of gastric ulcers by subcutaneous administration of indomethacin at a dosage of 35 mg/kg body weight after 24 hours of hunger. In a second series of experiments, at first was performed simulation of gastric ulcers, and then for 21 days, nanosilver solution at the concentration of 0.01 g/l was given to drink to the first group of rats (n=10), distilled water – to the second group (n = 10). Group of intact animals (n=9) which drank a distilled water within 21 day was used as a control. Parameters of elastase like activity, trypsin like activity, antitrypsin like activity, acid-stable inhibitors in serum and supernatant of gastric mucosa homogenate was determined with the help of enzymatic methods. Results. It has been found out that prophylactic oral application of nanosilver in modeling acute gastric ulcer contributed less pronounced activation of non-specific proteases and preservation in the gastric mucosa of the inhibitory capacity at a sufficiently high level. The therapeutic use of nanosilver solution in modeling acute gastric ulcer was accompanied by significant increase of proteinase inhibitors on the background of decreased activity of non-specific proteases at the local level. Prophylactic and therapeutic application of nanosilver solution is accompanied by decrease in morphological signs of inflammation in gastric mucosa. Conclusion. The results showed that nanosilver solution has the ability to block the development of ulcers in the stomach mucosa in the prophylactic and therapeutic application, which was confirmed by a statistically significant reduction in the degree of activation of proteolytic enzymes while increasing antiproteinases potential as well as the intensification of reparative processes of damaged tissue.