Role of the lymphatic drainage in change of cytokine activity of and neutrophil functions in rats after thermal skin burn

Abstract

Simultaneous complex investigation of cytokine activity — IL-1β, TNFα, IL-2, IL-4 and neutrophil functions in Wistar rat blood in norm and in dysfunction of lymphatic drainage after thermal skin burn 3А degree (10% of a body surface) has been performed. Blood serum cytokine levels were detected by flowing immunofluorescence method with using «Bio-Rad» test-system (USA). Neutrophil functions were estimated on ability to phagocytize heat-killed St. aureus and on spontaneous and stimulated (prodigiozan, heat-killed St. aureus) NBT reduction. Inhibition of the rise of IL-1β, IL-2 and TNFα activity and the increase of the IL-4 values in 2 times were found in the systemic circulation at the first week during dysfunction of skin lymphatic drainage. Neutrophil phagocytic activity was decreased in 1,4 times at 3 and 7 days, but spontaneous ability to reduce NBT was increased only on 3 day. The neutrophil phagocytic activity was normalized at the second week, but ability of these cells to reduce NBT and to respond on stimulation by prodigiozan or heat-killed St. aureus were decreased. The proinflammatory cytokines IL-1β, TNFα levels were increased at 30 day. Thus, two-phase change of cytokine activities with different biological effects and blood neutrophil functional activity caused by lymphatic drainage state was found.