Effect of krypton-oxygen mixture on the neurological status of rats after modelling of open traumatic brain injury

  • Victoria Vitalievna Antonova Federal Research and Clinical Center of intensive care medicine and rehabilitology, 25, p. 2, Petrovka str., Moscow, 107031, Russian Federation https://orcid.org/0000-0002-0819-7886
  • Dmitry Vladimirovich Kuidin Federal Research and Clinical Center of intensive care medicine and rehabilitology, 25, p. 2, Petrovka str., Moscow, 107031, Russian Federation https://orcid.org/0009-0008-2902-9962
  • Mikhail Valerievich Gabitov Federal Research and Clinical Center of intensive care medicine and rehabilitology, 25, p. 2, Petrovka str., Moscow, 107031, Russian Federation https://orcid.org/0009-0005-9615-6118
  • Ivan Alexandrovich Kryukov Federal Research and Clinical Center of intensive care medicine and rehabilitology, 25, p. 2, Petrovka str., Moscow, 107031, Russian Federation https://orcid.org/0000-0003-3121-2981
  • Ivan Valerievich Redkin Federal Research and Clinical Center of intensive care medicine and rehabilitology, 25, p. 2, Petrovka str., Moscow, 107031, Russian Federation https://orcid.org/0000-0001-7008-2038
  • Rostislav Alexandrovich Cherpakov Federal Research and Clinical Center of intensive care medicine and rehabilitology, 25, p. 2, Petrovka str., Moscow, 107031, Russian Federation https://orcid.org/0000-0002-0514-2177
  • Artem Nikolaevich Kuzovlev Federal Research and Clinical Center of intensive care medicine and rehabilitology, 25, p. 2, Petrovka str., Moscow, 107031, Russian Federation https://orcid.org/0000-0002-5930-0118
DOI: https://doi.org/10.48612/pfiet/0031-2991.2025.04.79-88
Keywords: krypton, noble gases, TBI, rats

Abstract

Introduction. Brain injury is one of the leading causes of death worldwide. There are no scientific studies on the effect of krypton on traumatic brain injury. The aim of the work was to study the effect of krypton-oxygen mixture on the neurological status of rats after modelling of open traumatic brain injury.

Methods. Experimental work was performed on male rats of Wistar line weighing 350-390 g (n=33). The animals were randomly divided into 3 groups depending on the volume of the interventions performed: 1. Sham-operated animals with inhalation of nitrogen-oxygen mixture (N2 70%/O2 30%), n=6 (Sham group); 2. control group of animals with TBI and inhalation of nitrogen-oxygen mixture (N2 70%/O2 30%), n=11 (TBI group); 3. experimental group with TBI and inhalation of krypton-oxygen mixture (Kr 70%/O2 30%), n=16 (TBI+iKr group). During the awakening period, the body temperature of the animals was maintained at 37±0.5oC using an infrared lamp. The simulation of traumatic brain injury was performed under intraperitoneal anaesthesia with chloral hydrate 300 mg/kg according to the method of dosed open brain contusion injury. Falsely operated animals also underwent drilling of a hole in the skull, but without impact. To assess the neurological status of laboratory rats, 2 tests were used: «Limb Placement test» and «Cylinder».

Results. The study of neurological deficit at 14-day observation using the «Limb Placement Test» revealed statistically significantly less neurological deficit on days 7 and 14 of the posttraumatic period in animals treated with krypton-oxygen mixture against the background of the transferred brain damage.

Conclusion. Inhalation of a krypton-oxygen mixture after traumatic brain injury resulted in improved neurological recovery in laboratory animals by reducing secondary brain damage. These data open new perspectives for further studies of krypton as a potential neuroprotector, which may become a new therapeutic agent in neuroresuscitation.

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Published
29-10-2025
How to Cite
Antonova V. V., Kuidin D. V., Gabitov M. V., Kryukov I. A., Redkin I. V., Cherpakov . R. A., Kuzovlev A. N. Effect of krypton-oxygen mixture on the neurological status of rats after modelling of open traumatic brain injury // Patologicheskaya Fiziologiya i Eksperimental’naya Terapiya (Pathological physiology and experimental therapy). 2025. VOL. 69. № 4. PP. 79–88.
Issue
Vol. 69 No. 4 (2025)
Section
Original research

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