Рathomorphology and pathomorphosis of ischemic infarction of the cerebral cortex in rats after the use of erythropoietin and laser radiation

Abstract

Relevance. Ischemic stroke is the most common (80% of cases) type of cerebral infarction with a high mortality rate, resulting from a critical decrease in cerebral blood flow - up to 20 ml / 100 g / min. This leads to dysfunction of neurons with preserved viability - the penumbra area (half-shadow), where cell death by apoptosis occurs in about 80 minutes. Thus, as a result of ischemic stroke, a central, irreversibly damaged infarction core and a potentially reversible, peripheral zone of ischemic half-shadow are formed, which is a target for therapy. In this regard, the search for new methods of pathogenetic therapy of ischemic stroke is an urgent task. Objective. This work was to study the combined effect of EPO and NLI on the neurological status, morphology of the lesion in experimental ischemic infarction of the cerebral cortex (EICI). Methods. Histological, immunohistochemical methods and statistical analysis were used. Morphometric studies included counting the number of damaged and intact neurons, small blood vessels, endothelial cells with FEGF expression, infarction area, and examined the neurological status. Results. A positive effect of erythropoietin in combination with low-intensity infrared laser radiation on the morphology of the experimental cerebral ischemia focus and neurological status in rats was established. On days 7-14-30, therapeutic pathomorphosis is recorded in the ischemic infarction focus: the number of intact neurons and capillaries increases, VEGF expression increases, the number of damaged neurons and infarction area decreases, and on day 14, the integral indicator of neurological status is completely restored. Conclusion. With the combined use of erythropoietin in a total dose of 15,000 IU/kg and infrared laser radiation with a wavelength of 970 nm, a power of 1 W in experimental IICGM in the stroke focus, a therapeutic pathomorphosis is recorded: on the 7th, 14th and 30th days, the number of neurons with chromatolysis and shadow cells, the area of ​​the infarction progressively decreases; the number of intact neurons, endothelial cells with VEGF expression, small blood vessels increases; from the 14th day, the integral indicator of the neurological status is completely restored