Effect of damage integrity rat brain synaptic membranes on the functional activity GABAₐ-receptor/Cl⁻-ionophore complex in the CNC

Abstract

Functional activity of the GABA_A-receptor/Cl-ionophore complex was investigated the muscimol-stimulated entry of the radioactive isotope ³⁶Cl⁻ in synaptoneurosomes in changing the structure and permeability of neuronal membranes. Integrity of the membranes was damaged by removal of Ca⁺² and Mg⁺² from the incubation medium and by the method of freezing-thawing synaptoneurosomes. In both cases, an increase in basal ³⁶Cl⁻ entry into synaptoneurosomes, indicating increased nonspecific permeability of neuronal membranes, and decreased activity the GABA_A-receptor/Cl⁻- ionophore complex. The conclusion about the relationship of processes damage neuronal membranes and reducing the inhibitory processes in the epileptic focus.