The response of placental decidual cells to treatment of chlamydiosis in pregnant women

Abstract

Aim. To evaluate the effect of antibiotics on placental decidual cells in the treatment of chlamydiosis during pregnancy. Methods. The study was performed on 6,000 decidual cells in different zones of placenta from mothers with uncomplicated pregnancy and chlamydiosis treated during pregnancy. The cells were stained with hematoxylin-eosin according to standard histological techniques. Method. The major and minor diameters of decidual cells and nuclei and the number of decidual cells per mm² of basal lamina and septa were determined using histostereometry. Based on the obtained values the nucleus-cytoplasm ratio and volumes of decidual cells, their cytoplasm and nuclei were calculated. Populations of stromal decidual cells of the basal lamina and septa were characterized. Variation curves of nucleus volume were constructed for decidual cells of the basal lamina and septa. Results. The antibiotic therapy affected primarily cells of the placenta central area. In this process, the cell proliferative status decreased toward the periphery of placenta where compensatory-adaptive mechanisms of decidual cells are weak. This was associated with impairment of essential immune responses in the placenta. The emergence of additional peaks in the variation curves of nucleus volume may indicate high mitotic activity and functional swelling of nuclei, probably due to cytotoxic effects of antibacterial drugs. Conclusion. The used regimen of antibiotic therapy for chlamydiosis in pregnant women exerted a minor beneficial effect. Results of this study can be used to develop new regimens for treatment of chlamydiosis during pregnancy with antibacterial drugs with a weaker effect on the nucleus apparatus of cells in the placental decidual tissue.